Jumat, 09 Maret 2012

Glutamin Meningkatkan IP Makrofag Terhadap S. aureus

EFEK GLUTAMIN DALAM MENINGKATKAN INDEKS FAGOSITOSIS MAKROFAG PERITONEUM TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR TERHADAP BAKTERI Staphylococcus aureus
(IN VITRO)
 
Yudistian, Syahroni. dr. Dewi Santosaningsih, Mkes. Dr. dr. Loeki Enggar Fitri, Mkes. SpParK. 2011. Medical Program, Faculty of Medicine, Brawijaya University.



Using antibiotics constantly on the control of bacterial infections of Staphylococcus aureus (S. aureus) are resulted in resistance. The use of methods other than antibiotics need to be developed, one of which is an increase in endurance. Glutamine is a non-essential amino acids material that shown to increase production of Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Nitric Oxide (NO), and cytokines required for activation of macrophage function. This study was designed to prove whether the administration of glutamine can enhance peritoneal macrophage phagocytosis index of white rat Rattus novergicus wistar strains against the bacteria S. aureus in vitro. This study used an experimental design laboratory, Randomized Post Test Only Control Group Design, using a rat strain wistar weight 250-300 grams total of 18 tails were divided into 2 groups: K (without the provision of glutamine), P (glutamine dose of 300 mg/kg/day). Treatment administered for 2 weeks. Macrophages isolated directly from mouse peritoneal macrophages. Staphylococcus aureus obtained from the collection of Microbiology Laboratory of the Brawijaya Univercity. Analysis of data by an independent T-test statistical test with 95% confidence level (α = 0.05). There are significant differences in phagocytic index between group K and group P (p = 0.010). Administration of glutamine dose 300 mg/kg/day can enhance peritoneal macrophage phagocytosis index of white rat Rattus novergicus wistar strains against the bacteria S. aureus in vitro.


Keywords: macrophage phagocytosis, S. aureus, glutamine, NADPH, NO, cytokine

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